Cat. No.
MABL-1607
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Saccharomyces cerevisiae
Clone No.
B2B4
From
Recombinant Antibody
Specificity
The antibody recognized a conformational epitope on the Heme-binding domain of Flb2 (residues 1-99). Specifically, the epitope consists of Asn30, residues 63 to 65 (GluPro-Leu) and 67 to 70 (Ala- Pro-Asn-Val), Asp72, Tyr74, and the two heme propionate groups.
Alternative Names
Cytochrome b2 mitochondrial; L-lactate dehydrogenase [Cytochrome]; L- lactate ferricytochrome C oxidoreductase; L-LCR
UniProt
P00175
Immunogen
The original antibody was generated by immunizing BALB/c mice with fully purified Flb.
Application Notes
ELISA indicated that the antibody, either the IgG or the Fab fragment, was able to recognize the heme-binding domain of flavocytochrome b2. (PMID: 8674650) For the Fab fragment, Kd values of 80 nM and 30 nM were determined in ELISA competition experiments and fluorescence quenching titrations, respectively. (PMID: 9521665)
Antibody First Published
Lé et al. Functional uncoupling between flavin and heme domains in flavocytochrome b2 by a monoclonal antibody. Biochem Soc Trans. 1996 Feb;24(1):16S. PMID:8674650
Note on publication
The paper describe the generation of the antibody B2B4, which inhibits the enzymatic activity of flavocytochrome b2. Further studies were carried out using either the purified divalent IgG1 or the monovalent Fab fragment of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
