Cat. No.
MABL-1617
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
F9
From
Recombinant Antibody
Specificity
This antibody is a general fluoroquinolone binder that likely binds to the antigen at the common pyridone-carboxylic acid pharmacophore.
Alternative Names
Quinoline monocarboxylic acid; antibiotic
UniProt
Immunogen
The original antibody was isolated from a human scFv phage display library by panning against biotinylated fluoroquinolone antibiotic.
Application Notes
This antibody was capable of binding the biotin analogue of fluoroquinolone in an ELISA. The binding affinity of the original scFv antibody to norfloxacin-biotin analogue was reported to be Kd = 1.58 µM. This antibody was used in the development of an ScFv biosensor for qualitative detection of antibiotics in livestock drinking water (PMID: 21142055),
Antibody First Published
Young Cha et al. Pharmacophore-based strategy for the development of general and specific scFv biosensors for abused antibiotics. Bioconjug Chem. 2011 Jan 19;22(1):88-94. PMID:21142055
Note on publication
Describes the development of a fluorescent biosensor system using antibodies for detection of fluoroquinolone antibiotics.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
