Cat. No.
MABL-1618
Application
functional assays, WB, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Chlamydomonas
Clone No.
61
From
Recombinant Antibody
Specificity
This antibody is specific for an amino acid sequence in the core protein of Flagellar membrane glycoprotein 1B/ FMG-1B. FMG-1B is a protein part of the flagella of Chlamydomonas.
Alternative Names
Flagella membrane glycoprotein 1B
UniProt
Q84X68
Immunogen
The original mouse version of this antibody was raised by immunizing mice with flagella from C.reinhardtii.
Application Notes
To study the distribution of the epitope to which this antibody binds. An ELISA was preformed on purified flagella from C.reinhardtii using the mouse version of this antibody (Bloodgood et al, 1986; pmid:3009491). Further to determine the unperturbed distribution of the mouse version of this antibody immunfluorescence was preformed on flagella from c.reinhardtii (Bloodgood et al, 1986; pmid:3009491). To determine relocation of surface glycoproteins in chlamydomonas flagella western blot was preformed on a mutant cell line of Chlamydomonas reinhardtii pf18 and LMJ.RY0402.100240 (Kamiya et al, 2018; pmid:30079834). To determine the transport of IFT trains and the interaction of FMG proteins with this. The mouse version of this antibody was used for to cover beads which were subsequently used to monitor IFT trains (Shih et al, 2013; pmid:23795295).
Antibody First Published
Bloodgood et al. Redistribution and shedding of flagellar membrane glycoproteins visualized using an anti-carbohydrate monoclonal antibody and concanavalin A J Cell Biol. 1986 May;102(5)15:071797-812. PMID:3009491
Note on publication
This antibody was generated and charatarized.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
