Cat. No.
MABL-1627
Application
therapeutic, ELISA, FC, IF
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
9H11
From
Recombinant Antibody
Specificity
This antibody binds human and mice FSH receptor. FSH receptor is a G protein-coupled receptor for follitropin, the follicle-stimulating hormone. Through cAMP production activates the downstream PI3K-AKT and ERK1/ERK2 signaling pathways.
Alternative Names
FSHR; FSH-R; Follicle-stimulating hormone receptor; Follitropin receptor; LGR1
UniProt
P23945
Immunogen
The original antibody was generated by immunizing Balb/c mice with an optimized DNA construct encoding human FSHR.
Application Notes
To test if the antibody dependent cellular cytotoxicity elicited by the mouse version of this antibody translated into an in vivo anti-tumor effect, mice were challenged with IDS -Defl29/Vegf-a-Fshr and treated with the mouse version of this antibody. The antibody was able to delay tumor progression. To further characterize the antibody its ability to bind cells expressing human and mouse FHSR, flow cytometry was performed using the mouse version of this antibody on A20 cells, ID8- Defl29/Vegf-a-Fshr cells and human ovarian cancer cell lines like Caov3, OVCAR3, TOV21 G and PC3 with physiological levels of FSHR. Isotype analysis of the antibody was done using ELISA. Finally immunofluorescence was performed on 293T cells transduced with human or murine FSHR using the mouse version of this antibody (WO2020033797).
Antibody First Published
Perales-Puchalt et al. WO2020033797
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
