Cat. No.
MABL-1628
Application
ELISA,
Isotype
Engineer antibody
Species Reactivity
Francisella tularensis, Francisella tularensis subsp. Holarctica
Clone No.
Ab52
From
Recombinant Antibody
Specificity
This antibody strongly binds the internal O-antigen (OAg) of the gram-negative bacterium Francisella tularensis (Ft).
Alternative Names
O-antigen of Francisella tularensis; O-antigen ligase family protein
UniProt
A0A0B6E216
Immunogen
The original antibody was generated by repeatedly immunizing BALB/c mice with live vaccine strain (LVS) and F. tularensis lipopolysaccharide admixed with CpG ODN 1826 (TCC ATG ACG TTC CTG ACG TT) (an oligodeoxynucleotide containing unmethylated CpG dinucleotides with fully phosphorothioate backbone.
Application Notes
This potent binder can be used to detect the O antigen of F. tularensis. The antigen specificity of this antibody was tested using ELISA. It is capable of binding to heat killed live vaccine strain and SchuS4 F. tularensis strains in an ELISA. This antibody was also capable of detecting the longer chains of the lipopolysaccharide ladder in a western blot assay (PMID： 21466282). Ab52 can be used as a reporter to monitor the response of vaccine recipients to protective B-cell epitopes of F. tularensis OAg (PMID： 22486311).
Antibody First Published
Roche et al. Characterization of monoclonal antibodies to terminal and internal o-antigen epitopes of Francisella tularensis lipopolysaccharide. Hybridoma (Larchmt). (2011); 30(1)： 19–28. PMID：21466282
Note on publication
Describes the generation of antibodies towards terminal and internal O-antigen epitopes of Francisella tularensis lipopolysaccharide.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
