Cat. No.
MABL-1630
Application
ELISA, WB
Isotype
Engineer antibody
Species Reactivity
Francisella tularensis
Clone No.
N62
From
Recombinant Antibody
Specificity
This antibody binds the non-reducing end of the O-antigen (OAg) of the gram-negative bacterium Francisella tularensis (Ft). The antibody N62 binds mainly to the terminal Qui4NFm sugar residue at the nonreducing end of OAg.
Alternative Names
O-antigen of Francisella tularensis; O-antigen ligase family protein; non- reducing end of F. tularensis OAg
UniProt
A0A0B6E216
Immunogen
The original antibody was generated by immunizing BALB/cJ mice intradermally with Francisella tularensis holarctica strain LVS (live vaccine strain) and later on a booster immunization with an outer membrane-enriched LVS preparation of F. tularensis.
Application Notes
This antibody binds the same epitope as clone FB11 and has a higher antigen-binding bivalent avidity than internally binding antiOAg mAb. This antibody is capable of detecting F. tularensis SchuS4 strain in an ELISA. It is also capable of agglutinating F. tularensis SchuS4 and live vaccine strain.
Antibody First Published
Lu et al. The binding sites of monoclonal antibodies to the non-reducing end of Francisella tularensis O-antigen accommodate mainly the terminal saccharide. Immunology. (2013); 140(3)：374-89. PMID：23844703
Note on publication
Describes the generation, characterization and in vivo efficacy of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
