Cat. No.
MABL-1644
Application
Blocking, functional assays, neutralization, WB, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
1G3
From
Recombinant Antibody
Specificity
This antibody is specific for human Gal-9 but exhibits cross-binding of murine Gal-9.
Alternative Names
Galectin-9; Gal-9; LGALS9; Ecalectin; Tumor antigen HOM-HD-21
UniProt
O00182
Immunogen
The original antibody was generated by immunizing BALB/c mice with a recombinant c- terminus galectin-9 (residues 191 to 355 of the galectin-9 long isoform).
Application Notes
The binding and binding specificity of the original format of this antibody to c-terminus Gal-9 were assessed via ELISA. Epitope mapping was also done using ELISA. Furthermore, this antibody was successfully used in WB and IHC assays (Barjon et al., 2012; PMID: 22805533). An scFv-CAR version of this antibody with therapeutic potential was developed and described (US20230295564A1). This antibody was successfully used to neutralize LGALS9 in a study focused on pancreatic neoplastic lesions (Quilbe et al., 2023; PMID: 38098486). This antibody was used in a T lymphocyte proliferation assay, specifically in assessing the immunomodulatory properties of extracellular vesicles (C15SEVs) derived from nasopharyngeal carcinoma and recombinant Galectin-9S. In this assay, the antibody was used to block the impact of C15SEVs and recombinant Galectin-9 on peripheral blood mononuclear cells (Lefebvre et al., 2023; PMID: 38117000).
Antibody First Published
Barjon et al. A novel monoclonal antibody for detection of galectin-9 in tissue sections: application to human tissues infected by oncogenic viruses. Infect Agent Cancer. 2012 Jul 17;7(1):16. PMID:22805533
Note on publication
The original publication presents the development of monoclonal antibodies targeting galectin-9 and focuses on the development and characterization of a novel monoclonal antibody, 1G3, with a particular emphasis on its application in improving the detection and understanding of galectin- 9 expression in clinical samples.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
