Cat. No.
MABL-1647
Application
in vivo, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
FF06
From
Recombinant Antibody
Specificity
The antibody binds to human galectin-2. Galectin-2 is a monocyte-expressed carbohydrate- binding lectin, which plays a role in cancer, myocardial infarction, immune response, and gastrointestinal tract diseases.
Alternative Names
Gal-2; Beta-galactoside-binding lectin L-14-II; HL14; Lactose-binding lectin 2; S-Lac lectin 2
UniProt
P05162
Immunogen
The original antibody was isolated from the antibody library after two rounds of panning against human galectin-2.
Application Notes
The scFv fragment was able to bind the recombinant human galectin-2 protein in ELISA experiments. Immunofluorescence analysis on the colon biopsy samples from a healthy donor was performed. The scFv fragment showed significant staining surrounding the crypts of the intestinal tissue. Immunofluorescence analysis on the human colorectal adenocarcinoma cell line LS174T was performed in order to verify the ability of the scFv fragment to recognize natively expressed galectin-2 antigen. The ScFv fragment showed strong cytoplasmic staining. Immunofluorescence analysis on human biopsy samples of Ulcerative Colitis was performed. A stronger intensity signal in the colonic crypts region can be detected in the sections stained with the scFv fragment when compared to the signal observed with an irrelevant antibody. The autoradiography on the intestinal tract of a mouse having colitis has been performed in order to verify the ability of the scFv fragment to recognize the galectin-2 antigen in vivo. A stronger intensity signal could be detected in the colon of the mouse injected with the scFv fragment when compared to the signal in a control mouse injected with an irrelevant antibody (US20190309060).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
