Cat. No.
MABL-1649
Application
functional assay, Block, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human, Rhesus Monkey, Cynomolgus Monkey
Clone No.
B1.1
From
Recombinant Antibody
Specificity
This antibody is specific for the human TCR delta and gamma chains. The T-cell receptor (TCR) is a molecule found on the surface of T cells, or T lymphocytes, that is responsible for recognizing fragments of antigen as peptides bound to major histocompatibility complex (MHC) molecules. On activation through antigen presenting cells (APCs), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. TCR part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response.
Alternative Names
TCR γδ; γδ TCR; TCR GD; GD TCR; TCRg/d; T-cell surface glycoprotein CD3 delta chain; CD3D; T3D; T-cell receptor T3 delta chain; T-cell surface glycoprotein CD3 gamma chain; CD3G; T-cell receptor T3 gamma chain; CD3g; T3G; B1
UniProt
P04234; P09693
Immunogen
Antigen-specific T cell blasts to poly-18, a polypeptide antigen of defined sequence and conformation, were generated from lymph nodes of antigen primed BALB/cCr mice. These blasts were fused with the BW5147 thymoma to obtain anti-poly-18-reactive T cell hybridomas from which the original antibody was generated.
Application Notes
This antibody can be used for the flow cytometric analysis of human peripheral blood cells (PMID: 12121897; 23447017; 25777993; 28223321). This antibody was used to block human γδ T cell receptor and prevent the human anti-porcine γδ T cell response (PMID: 11502983; 28713391). This antibody was also used to block the γδ T cell receptors in in-vitro tumor-killing assays (PMID: 21633088). This antibody was used for the immunohistochemical analysis of frozen tissue samples of parapsoriasis, mycosis fungoides and inflammatory dermatoses (PMID: 21479436). This antibody was also used for the immunohistochemical and indirect immunofluorescence staining of γδ T cells in breast cancer and normal tissues (PMID: 23959855).
Antibody First Published
Fotedar et al. Fine specificity of antigen recognition by T cell hybridoma clones specific for poly-18: a synthetic polypeptide antigen of defined sequence and conformation. J Immunol. 1985 Nov;135(5):3028-33. PMID:2413110
Note on publication
Describes the epitope characterization of T cell hybridomas specific for Poly-18.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
