Cat. No.
MABL-1655
Application
functional assays, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
AKH11
From
Recombinant Antibody
Specificity
This antibody is specific for gangliosides GM3 (N-acetylneuraminic acid), GT1b, GD3, GD1a, and GQ1b but does not react with other glycolipids such as lactosylceramide, GM3 (N-glycolylneuraminic acid), GM2, GM1, GD2, GD1b, 9-o-Ac-GD3, SSEA-1, or sialylparagloboside.
Alternative Names
GM3 (N-acetylneuraminic acid); GT1b; GD3; GD1a; GQ1b
UniProt
Immunogen
The original antibody was generated by preparing and establishing an EBV-transformed human cell line from lymphocytes obtained from a melanoma patient.
Application Notes
The specificity of the original antibody (human IgM) was assessed using ELISA, revealing that it reacted with gangliosides in the following order: GM3 (N-acetylneuraminic acid) > GT1b > GD3 > GD1a > GQ1b. A recombinant version of the antibody was generated, and its biological activity was evaluated. CDC activity tests demonstrated efficacy against MeWo, M12, M14, and G-361 melanoma cells, while showing no significant CDC activity against human normal skin fibroblasts, human adenocarcinoma, human neuroblastoma, or Vero cells. These findings confirm that the recombinant antibody retains the biological activities of the original AKH11 antibody (EP0492409A1).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
