Cat. No.
MABL-1656
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Ganoderma
Clone No.
12 A
From
Recombinant Antibody
Specificity
mAb 12 A was shown to react with ganoderic acid A using indirect and competitive ELISAs (Sakamoto et al, 2016). It was also shown that mAb 12 A did not cross react significantly with 32 other types of Ganoderma triterpenoids. Ganoderic acid A is one of the main bioactive triterpenoids in the Ganoderma genus of medicinal mushrooms, traditionally used in medicine.
Alternative Names
GAA; 12A; 12-A
UniProt
Immunogen
BALB/c mice were immunised by injection of ganoderic acid A-BSA conjugates. Splenocytes were obtained from immunised mice and fused with SP2/0 murine myeloma cells to generate hybridomas.
Application Notes
mAb 12 has been used in both indirect and competitive ELISAs, using 33 different types of Ganoderma triterpenoid including ganoderic acid A (Sakamoto et al, 2016). More recently, mAb 12 has been reformatted as a scFv fragment and applied in ELISAs for detection of ganoderic acid A (Sakamoto et al, unpublished).
Antibody First Published
Sakamoto et al. Detection of Ganoderic Acid A in Ganoderma lingzhi by an Indirect Competitive Enzyme-Linked Immunosorbent Assay. Planta Med. 2016 May;82(8):747-51. PMID:27093250
Note on publication
Describes the generation of the anti-ganoderic acid A mAb 12 A, and preliminary characterisation of its reactivity using Ganoderma triterpenoids by ELISAs.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
