Cat. No.
MABL-1657
Application
WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
lesion-scFv
From
Recombinant Antibody
Specificity
This antibody is specific for human GAPDH.
Alternative Names
GAPD; Glyceraldehyde-3-phosphate dehydrogenase; Peptidyl-cysteine S- nitrosylase GAPDH; EC:1.2.1.12; EC:2.6.99.
UniProt
P04406
Immunogen
The original antibody was generated by assembling immunoglobulin VH and VL genes from IgA-positive B cells located in lesions of an MS brain.
Application Notes
This antibody's original format (human scFv) was used in an ELISA and WB assay (Kolln et al., 2006; PMID: 17015754).
Antibody First Published
Kolln et al. Triosephosphate isomerase- and glyceraldehyde-3-phosphate dehydrogenase-reactive autoantibodies in the cerebrospinal fluid of patients with multiple sclerosis. J Immunol. 2006 Oct 15;177(8):5652-8. doi: 10.4049/jimmunol.177.8.5652. PMID:17015754
Note on publication
The original publication investigates the presence of autoantibodies, including single-chain variable fragment antibodies (scFvs) generated from clonal B cells in the cerebrospinal fluid and lesions of multiple sclerosis (MS) patients, targeting the glycolytic enzymes triosephosphate isomerase (TPI) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH).
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
