Cat. No.
MABL-1658
Application
functional assay, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
XPA067.06
From
Recombinant Antibody
Specificity
This antibody is specific for gastrin 17. Gastrin is a peptide hormone that stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine. It has also been implicated in the development or maintenance of various types of cancer, such as pancreatic and stomach carcinoma.
Alternative Names
Gastrin component III; Gastrin-17; G17
UniProt
P01350
Immunogen
The original antibody was generated by panning three against human antibody phage display libraries using a short 10 amino acid peptide from the N terminal portion of Gastrin 17.
Application Notes
To determine the efficacy of gastrin neutralization by these antibodies in vitro, a flow cytometric measurement of intracellular calcium flux was performed using the human IgG1 version of this antibody on a CCK2R overexpressing cell line (AGS-TR). This antibody showed a 70%-80% neutralization in Ca flux in cells stimulated with gastrin (US8278421) (Hsu et al, 2008; pmid:18589401). Further the binding affinity of the human version of this antibody was determined using surface plasmon resonance and affinity constants were calculated from the kinetic rate constants for antibody binding to biotinylated peptide immobilized on a Streptavidin sensor chip (US8278421) (Hsu et al, 2008; pmid:18589401). The human version of this antibody has shown the ability to increase the stomach pH of mice to pH 4 in combination with human gastrin and famotidine. The pH with only human gastrin and famotidine was pH 2.5 (US8278421) (Hsu et al, 2008; pmid:18589401). Screening of gastrin positive antibody clones was done using ELISA (Hsu et al, 2008; pmid:18589401). Inhibition of gastrin activity has potential for therapeutic use as a suppressor of acid secretion as well as an inhibitor of gastrin-responsive tumors (Hsu et al, 2008; pmid:18589401).
Antibody First Published
Hsu et al. Development of XPA067.06, a potent high affinity human anti- gastrin monoclonal antibody. Biochem Pharmacol. 2008 Aug 1;76(3)340-52. PMID:18589401
Note on publication
Describes the generation of high affinity antibody towards gastrin hormone and explores its therapeutic potential to be used as a suppressor of acid secretion as well as an inhibitor of gastrin-responsive tumor.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
