Cat. No.
MABL-1661
Application
functional assays, SPR, ELISA, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
3B2-3C9
From
Recombinant Antibody
Specificity
This antibody is specific for the human disialoganglioside GD2, a surface antigen highly expressed on human neuroblastoma cells. The antibody showed high specificity for GD2 without cross- reactivity to other similar gangliosides like GD1a, GM2, and GM3. Surface plasmon resonance and immunostaining techniques confirmed its strong and specific binding to GD2.
Alternative Names
AB527
UniProt
Q9UI17
Immunogen
The original antibody was derived from Epstein-Barr Virus transformed human lymphoblast cells.
Application Notes
Affinity testing of the original antibody (human IgM) to GD2 via SPR revealed a Kd of 4.5 nM. The antibody effectively labeled GD2-expressing cells in both flow cytometry (FC) and immunofluorescence (IF) assays. ELISA testing across different GD2-expressing cell lines (M14, LLC-MK2, and EL4) showed a high binding affinity for M14 and LLC-MK2 (0.13 ± 0.02 nM and 0.15 ± 0.04 nM, respectively), compared to EL4 (0.93 ± 0.16 nM). A biotinylated version of the antibody was used to stain various melanoma cell lines in an immunohistochemistry (IHC) assay. GD2-positive cells (M14) demonstrated strong staining, while GD2-negative lines (MG1055, RPMI7951, and JS0592) exhibited minimal or no staining, confirming the antibody's specificity. Additionally, the antibody was tested in an IHC panel of 35 normal human tissues to assess cross-reactivity, with most normal tissues showing no significant high- affinity staining, indicating minimal cross-reactivity and specificity toward GD2-expressing cells. The antibody successfully mediated complement-dependent cytotoxicity (CDC) on multiple GD2-positive melanoma cell lines (M14, LF0023, M101, M18, and M10VAC). Furthermore, the IgM version of the antibody exhibited superior CDC activity compared to a human IgG1 isotype-switched version (US8507657B2).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
