Cat. No.
MABL-1664
Application
IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
3D2
From
Recombinant Antibody
Specificity
The original antibody is specific for GD3, a tumor-associated antigens.
Alternative Names
disialogangliosides GD3
UniProt
Immunogen
The original antibody was originated by immunizing mice with multi-antigen peptides GD3P4.
Application Notes
The original format of the antibody was assessed by immunostaining on a thin-layer chromatography plate (ITLC) using a ganglioside fraction purified from human melanoma tumors. It bound specifically to GD3 and not to any other tested ganglioside. The binding specificity of the original format of the antibody was also investigated using bovine brain ganglioside fraction, where it slightly bound to GT1b. The original format of the antibody bound to the human melanoma SK MEL-28 cells (approximately 14% of the cells were recognized). The original format of the antibody was used for immunostaining of tumor tissue (Popa et al., 2006; PMID： 16458892).
Antibody First Published
Popa et al. GD3-replica peptides selected from a phage peptide library induce a GD3 ganglioside antibody response. FEBS Lett. 2006 Feb 20;580(5)：1398-404. doi： 10.1016/j.febslet.2006.01.063. Epub 2006 Jan 30. PMID：16458892
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
