Cat. No.
MABL-1665
Application
IHC
Isotype
Engineer antibody
Species Reactivity
Rat, Mouse
Clone No.
Ha1
From
Recombinant Antibody
Specificity
The original antibody binds to the disialylated gangliosides GD1b, GD3, GT1b, and GQ1b and weakly to GM3 and GD1a. It does not bind to structurally dissimilar glycolipids, including GM1, GM2, globoside, and sulphatide.
Alternative Names
disialylated gangliosides; GD1b; GT1b; GQ1b; GD3
UniProt
Immunogen
The original antibody was generated from peripheral blood B cells of an affected patient.
Application Notes
The original format of the antibody bound to a wide array of structures in the rodent peripheral nervous system, including dorsal root ganglia, peripheral nerve axonal profiles, compact myelin, nodes of Ranvier, motor end plates, and muscle spindles. In both rat and mouse dorsal root ganglia, the original format of the antibody stained the plasma membrane and cytoplasm of neurons. The antibody staining extended into the axonal lumen of myelinated nerve fibers. In teased fibers from mouse sciatic nerve, antibody-labeled compact myelin exposed at points of damage, created during the teasing process, and labeled axons protruding beyond ruptured myelin sheaths. It also labeled nodes of Ranvier in some intact teased fibers. In both mouse and rat, the original format of the antibody strongly labeled capillaries within the nerve bundle. In the rat muscle spindle, the antibody bound strongly to a region immediately adjacent to the intrafusal fibers, corresponding to the area defined by the internal capsule. The original format of the antibody also bound in the region of the neuromuscular junction in both mouse and rat muscle, as demonstrated by colocalization of Ha1 with BTx, a specific ligand for postsynaptic acetylcholine receptors (Willison et al., 1996; PMID： 8636426).
Antibody First Published
Willison et al. A Somatically Mutated Human Antiganglioside IgM Antibody that Induces Experimental Neuropathy in Mice Is Encoded by the Variable Region Heavy Chain Gene, V1-18. J Clin Invest. 1996 Mar 1;97(5)：1155-64. doi： 10.1172/JCI118529. PMID：8636426
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
