Cat. No.
MABL-1669
Application
IP, WB, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
GsnVHH 13
From
Recombinant Antibody
Specificity
This antibody recognizes Ca2+ activated gelsolin. This antibody specifically recognizies and epitope in the G4-G5 domain of gelsolin.
Alternative Names
GSN; AGEL; ADF; Actin-depolymerizing factor; Brevin
UniProt
P06396
Immunogen
The original antibody was generated by immunizing a llama subcutaneously with human recombinant gelsolin.
Application Notes
Gelsolin is a multifunctional actin-binding protein that controls cellular actin organization and migration. The original VHH version of this antibody binds calcium activated gelsolin with a Kd of ~10 nM. The specificity of the antibody towards gelsolin was confirmed with ELISA. GsnVHH 13 expressed as intrabodies immunoprecipitate endogenous gelsolin. His-tagged GsnVHH 13 can be used to identify gelsolin in a western blot assay. This antibody can trace gelsolin in membrane ruffles of EGF- stimulated MCF-7 cells and delay cellmigration without affecting F-actin severing/capping oractin nucleation activities by gelsolin as demonstrated by immunofluorescense staining. (PMID：20140750).
Antibody First Published
Abbeele et al. A llama-derived gelsolin single-domain antibody blocks gelsolin-G-actin interaction. Cell Mol Life Sci. 2010 May;67(9)：1519-35. PMID：20140750
Note on publication
Describes the generation, characterization, epitope mapping and crystal structure of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
