Cat. No.
MABL-1670
Application
neutralization, SPR, X-ray crystallography, ELISA
Isotype
Engineer antibody
Species Reactivity
Japanese Encephalitis Virus
Clone No.
2H4
From
Recombinant Antibody
Specificity
The antibody is specific for JEV. It binds a quaternary epitope that span across three adjacent envelope proteins. The antibody does not cross react with E protein from dengue (DENV1 and DENV2), Zika (ZIKV) and yellow fever (YFV) viruses.
Alternative Names
JEV; E protein; Envelope protein E
UniProt
P32886
Immunogen
The original antibody was generated by immunizing mice with JEV.
Application Notes
The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody was used in indirect immunofluoresenceThe antibody could neutralize the virus in mice (Zhang et al., 1989; PMID： 2557383). Surface plasmon resonance experiments showed that the antibody binds to the JEV rE with a high affinity of 2.9nM. The antibody could block attachment of the virus to its receptor and also prevent fusion of the virus. Cell-based neutralizing assays (in Vero cells) revealed that the antibody has strong neutralizing activities： a 50% neutralizing concentration value of 1.2 ng ml-1 against JEV (P3 strain). The antibody protected 100% of mice from lethal infection of JEV and led to complete clearance of JEV from the brains of mice, indicating its potential as a therapeutic agent. The structure of the Fab fragment of the antibody was determined by X-ray and of the Fab in complex with JEV by Cryo-EM (Qiu et al., 2018; PMID： 29379207).
Antibody First Published
Zhang et al. Passive protection of mice, goats, and monkeys against Japanese encephalitis with monoclonal antibodies J Med Virol. 1989 Oct;29(2)：133-8. doi： 10.1002/jmv.1890290211. PMID：2557383
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
