Cat. No.
MABL-1681
Application
FC
Isotype
Engineer antibody
Species Reactivity
Aequorea victoria (Water jellyfish, Mesonema victoria)
Clone No.
Clone 1
From
Recombinant Antibody
Specificity
This antibody binds green fluorescent protein.
Alternative Names
Green fluorescent protein
UniProt
P42212
Immunogen
The original antibody was isolated from a nanobody library derived from a llama immunized with green fluorescent protein using yeast surface display platform.
Application Notes
The binding characterization of this antibody for green fluorescent protein was done using flow cytometry. The binding affinity of this antibody for GFP was calculated to be Kd= 2.6nM (PMID： 19861136).
Antibody First Published
Ryckaert et al. Isolation of antigen-binding camelid heavy chain antibody fragments (nanobodies) from an immune library displayed on the surface of Pichia pastoris. J Biotechnol. 2010 Jan 15;145(2)：93-8. PMID：19861136
Note on publication
Describes the use of a yeast surface display platform for rapid discovery of conformationally selective nanobodies.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
