Cat. No.
MABL-1682
Application
in vitro, in vivo, IF
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
GBP1 (Enh)
From
Recombinant Antibody
Specificity
This antibody is specific for GFP.
Alternative Names
Green fluorescent protein; GFP Binding Protein 1; Enhancer
UniProt
P42212
Immunogen
The original antibody was generated by immunizing an alpaca with GFP.
Application Notes
The Enhancer (Enh) anti-GFP nanobody, also known as GFP binding protein 1 (GBP1), was specifically used for manipulating protein conformation in vitro and in vivo; this nanobody caused a fourfold fluorescence enhancement by stabilizing the deprotonated, negatively charged state of the GFP chromophore, resulting in increased fluorescence intensity (Kirchhofer et al., 2010; PMID： 20010839).
Antibody First Published
Kirchhofer et al. Modulation of protein properties in living cells using nanobodies Nat Struct Mol Biol. 2010 Jan;17(1)：133-8. doi： 10.1038/nsmb.1727. Epub 2009 Dec 13 PMID：20010839
Note on publication
The original publication explores the use of camelid-derived nanobodies to modulate the conformation and spectral properties of green fluorescent protein (GFP) in living cells, revealing the potential for manipulating and studying protein conformations using nanobodies.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
