Cat. No.
MABL-1683
Application
crystallization, in vitro
Isotype
Engineer antibody
Species Reactivity
Aequorea victoria (Water jellyfish, Mesonema victoria)
Clone No.
Minimizer
From
Recombinant Antibody
Specificity
The antibody is specific for green fluorescent protein. The antibody interacts with β-strands 6 and 7 of GFP.
Alternative Names
Green fluorescent protein; MI; GBP4
UniProt
P42212
Immunogen
The original antibody was generated by immunizing camels with GFP. A phage display library was constructed and the antibody was selected by panning against GFP.
Application Notes
The antibody could reversibly reduce GFP fluorescence by a factor of 5 in vitro. A comparable fluorescence modulation was also observed after addition of the antibody to soluble cell extract derived from human embryonic kidney (HEK) 293T cells expressing eGFP. The crystal structure of the GFP–antibody complex was determined. The dissociation constant of the antibody was measured (Kd= 0.45 nM) (Kirchhofer et al., 2010; PMID： 20010839). Fluorescence modulation in living cells was also observed after the addition of the antibody to HeLa cells expressing GFP (Schmidthals et al., 2010; PMID： 20372881).
Antibody First Published
Kirchhofer et al. Modulation of protein properties in living cells using nanobodies. (2010) Nat Struct Mol Biol 17： 133-138 PMID：20010839
Note on publication
The original paper describes the generation and characterization of the antibody
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
