Cat. No.
MABL-1686
Application
NTRL, WB, FC, IHC
Isotype
Engineer antibody
Species Reactivity
KSHV
Clone No.
YC15
From
Recombinant Antibody
Specificity
YC15 was shown to bind ectopically-expressed gH on transfected cell lines, as well as neutralise infection by KSHV virions (unpublished observations). Kaposi's sarcoma is a rare type of cancer, characterised by neoplasmic growth, often associated with AIDS.
Alternative Names
Glycoprotein H; Kaposi's sarcoma-associated herpesvirus glycoprotein H; KSHV glycoprotein H; KSHV gH; KSHV envelope glycoprotein H; Envelope glycoprotein H
UniProt
F5HAK9
Immunogen
Antibodies were produced from a murine hybridoma.
Application Notes
The YC15 variable domain sequences were cloned and redesigned into the scFv format (Cai & Berger, 2011), and further fused to PE38, a construct of Pseudomonas aeruginosa exotoxin A which is lacking the N-terminal cell adhesion domain. In this format, YC15 showed specific dose-dependent binding to surface-expressed gH using flow cytometry. As an immunotoxin with the fused P. aeruginosa PE38 construct, YC15 facilitated potent cytotoxic activity based on recognition of surface gH epitopes.
Antibody First Published
Cai & Berger. An immunotoxin targeting the gH glycoprotein of KSHV for selective killing of cells in the lytic phase of infection. Antiviral Res. 2011 Jun;90(3)：143-50. PMID：21440007
Note on publication
Describes the reformatting of YC15 variable domains (obtained in an unpublished study) as a YC15-scFv-PE38 immunotoxin. The gH-binding properties of YC15 were shown to be retained.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
