Cat. No.
MABL-1689
Application
neutralizing, WB, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Epstein-Barr virus
Clone No.
CL40
From
Recombinant Antibody
Specificity
The antibody binds to an epitope in gH D-II that overlaps substantially with a region observed to interact with the gp42 HP. In particular the antibody interacts with gH D-II using light chain contacts composed of L-CDR3 with the N-terminal end of gH:2α-7 (helix 7 in gH:D-II), and L-CDR1 with the loop connecting gH:2α-4 and gH:2α-5. Key residue interactions include hydrogen bonds between gH:D284 and L- CDR3:Y94, gH:E286 and L-CDR3:N92, and the backbone of gH:V243 and L-CDR1:N32. There is also minimal lateral contact with gH D-III between L-CDR1: Q27 and the C-terminal end of gH:3α-6, and VL:D1 with gH:N508 on the other side. The antibody heavy chain contacts with gH include H-CDR2:D52 and H- CDR2:D55, forming salt bridges with gH:R184, and backbone H-bonds between H-CDR3: L100 and H- CDR3:L101 with gH:H239.
Alternative Names
Envelope glycoprotein H; Envelope glycoprotein L
UniProt
P03231; P03212
Immunogen
Application Notes
The antibody blocks EBV-mediated membrane fusion with both B cells and epithelial cells and it retains its inhibitory activity as a monomeric Fab. Flow cytometry cell counts showed that Fab fragment inhibits virus entry. Neutralization experiments with epithelial cells using an EBV-GFP reporter virus and purified Fab were performed. Fluorescence images obtained at 24 h postinfection and flow cytometry cell counts showed that the Fab inhibits virus entry. Fab inhibition of cell–cell fusion and EBV infection exhibited similar concentration dependence. (PMID： 28939750) The cell-surface expression of gHgL was evaluated using the antibody by CELISA. (PMID： 27929061)
Antibody First Published
Sathiyamoorthy et al. Inhibition of EBV-mediated membrane fusion by anti- gHgL antibodies. Proc Natl Acad Sci U S A. 2017 Oct 10;114(41)：E8703-E8710. PMID：28939750
Note on publication
The authors studied the structure and mechanism of anti-gHgL antibody CL40 that blocks membrane fusion with both B cells and epithelial cells.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
