Cat. No.
MABL-1690
Application
neutralizing, WB, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Epstein-Barr virus
Clone No.
CL59
From
Recombinant Antibody
Specificity
The antibody binds an epitope in gH D-IV, proximal to the viral membrane and distant from known receptor binding sites. In particular, the antibody binds to the back side of gH D-III/D-IV close to gH D- III residues 494–503 (loop between 3α-8 and 3α-9), 456–468 (3α-6 helix), and 406–415 (loop between 3α-3 and 3α-4), and gH D-IV loop residues 645–656 (loop between 4α-1 and 4β-9), 623–626 (loop between 4β-7 and 4β-8), and 568–577 (loop between 4β-3 and 4β-4).
Alternative Names
Envelope glycoprotein H; Envelope glycoprotein L
UniProt
P03231; P03212
Immunogen
Application Notes
The antibody blocks EBV-mediated membrane fusion with both B cells and epithelial cells. Flow cytometry cell counts showed that Fab fragment inhibits virus entry. Neutralization experiments with epithelial cells using an EBV-GFP reporter virus and purified Fab. Fluorescence images obtained at 24 h postinfection and flow cytometry cell counts showed that the Fab inhibits virus entry. The Fab exhibited ∼50% inhibition of EBV infection at 150 nM, where no inhibition of cell–cell membrane fusion was observed. (PMID： 28939750) The cell-surface expression of gHgL was evaluated using the antibody by CELISA. (PMID： 27929061)
Antibody First Published
Sathiyamoorthy et al. Inhibition of EBV-mediated membrane fusion by anti- gHgL antibodies. Proc Natl Acad Sci U S A. 2017 Oct 10;114(41)：E8703-E8710. PMID：28939750
Note on publication
The authors studied the structure and mechanism of anti-gHgL antibody CL59 that blocks membrane fusion with both B cells and epithelial cells.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
