Cat. No.
MABL-1696
Application
therapeutic, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human, Rhesus Monkey
Clone No.
2D3
From
Recombinant Antibody
Specificity
This antibody binds human and rhesus monkey GITR (TNFRSF18; CD357) and does not cross react with mouse GITR. This protein acts as a receptor for TNFSF18, which is involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death.
Alternative Names
CD357; TNFRSF18; AITR; GITR; Tumor necrosis factor receptor superfamily member 18; Activation-inducible TNFR family receptor; Glucocorticoid-induced TNFR-related protein; 2D3-mHvKv
UniProt
Q9Y5U5
Immunogen
The original antibody was generated by immunizing 6-8 weeks old female BALB/c mice with his-tagged human GITR protein.
Application Notes
The initial binding characterization of the antibody was done using ELISA. The binding reactivity of this antibody to human, mouse and rhesus monkey GITR was determined using flow cytometry. This antibody was shown to cross react with human and rhesus monkey GITR and not mouse GITR. The binding affinity of the original mouse IgG1 antibody for human GITR was determined using surface plasmon resonance and was found to be Kd= 7.75X 10^(-10)M. The anti-tumor effect of this antibody was tested in vivo in a mouse colon cancer model. Murine MC-38 cells (colon adenocarcinoma cells) were injected subcutaneously in B-hGITR mice and treated with this antibody. It was reported that this antibody showed good tumor inhibitory effect (CN111763257).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
