Cat. No.
MABL-1695
Application
neutralize, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Gloydius intermedius (Central Asian pit viper, Agkistrodon intermedius)
Clone No.
N20 (3C12)
From
Recombinant Antibody
Specificity
This antibody binds the gintexin A type PLA2 protein found in the venom of Gloydius intermedius. Gintexin A is Asn-49 type PLA2 isomer that has no enzymatic activity and neurotoxicity. Gentexin is a heterodimer comprising gintexin A and gentexin B. It is estimated that, after the venom enter the blood system GintexinA acts as a "director" to guide the neurotoxin heterodimer to bind to the membrane receptor at the phrenic nerve NMJ synapse, and then Gintexin B exerts neurotoxicity (blocks the diaphragm NMJ synaptic transmission and stops breathing).
Alternative Names
Gintexin A; PLA2; phospholipase 2; GA-3C12
UniProt
A0A096XPP4
Immunogen
The original antibody was prepared by immunizing BALB/c mice with attenuated Gintexin antigen purified from the crude venom of Gloydius intermedius.
Application Notes
This antibody was capable of recognizing attenuated gintexin A of Gloydius intermedius and a fusion protein containing gintexin A in a western blot assay. The binding affinity of this antibody for gintexin A was determined using ELISA. 100 μg this antibody can neutralize at least 2×LD50 of Agkistrodon venom. In vivo, the neutralizing activity of this antibody is reported to be about 21.1 LD50/mg. The sensitivity of HRP-N20 antibody for immunospot detection of Agkistrodon intermedius venom is 12.5 ng/mL.
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
