Cat. No.
MABL-1694
Application
in vivo, WB, IF
Isotype
Engineer antibody
Species Reactivity
Rat, Human
Clone No.
TA10
From
Recombinant Antibody
Specificity
This antibody is specific for a 79 amino acid long region between amino acids 1462 to 1540 of the full human sequence (corresponding to amino acids 1399 to 1478 in the rat homologue) in Giantin, a Golgi matrix protein.
Alternative Names
Golgin subfamily B member 1; 372 kDa Golgi complex-associated protein; GCP372; Macrogolgin
UniProt
Immunogen
The original antibody was generated via phage display from the Griffin.1 library by screening against Rat liver Golgi (RLG) complexes derived from rat liver.
Application Notes
The original format of this antibody (scFv) was successfully used in a Western blot and for in vitro immunofluorescence straining of Giantin in HeLa cells. siRNA knock-down of Giantin reduced TA10 staining, confirming its specificity. Furthermore, an in vivo expressed TA10-GFP construct successfully stained HeLa cells (Nizak et al., 2003; PMID： 14617357). The scFv-Fc fusion version of this antibody — hTA10 — detected Giantin when cells were fixed by paraformaldehyde (Moutel et al., 2009; PMID： 19245715). Precise characterization of the TA10 target was achieved; a region of 79 amino acids, representing less than 2.5% of the entire protein, was determined as its binding site (Vielemeyer et al., 2010; PMID： 20727208).
Antibody First Published
Nizak et al. Recombinant antibodies against subcellular fractions used to track endogenous Golgi protein dynamics in vivo Traffic. 2003 Nov;4(11)：739-53. doi： 10.1034/j.1600- 0854.2003.00132.x PMID：14617357
Note on publication
The original publication demonstrates the use of recombinant antibodies selected through phage display against subcellular fractions.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
