Cat. No.
MABL-1710
Application
IP, WB, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
10F7MN
From
Recombinant Antibody
Specificity
The antibody recognizes the N and M-type of glycophorin A.
Alternative Names
CD235a; Glycophorin-A; MN sialoglycoprotein; PAS-2; Sialoglycoprotein alpha; Glycophorin A, type M and type N
UniProt
P02724
Immunogen
The antibody was generated by immunizing mice with Glycophorin A.
Application Notes
The Fab fragment recognized glycophorin a on the surface of erythrocytes by immunoprecipitation or western blotting techniques. The number of binding sites and dissociation constants of the Fab' fragment was determined (n = 4.66 × 10^5 sites/erythrocyte, Kd =9.5 × 10^-8 M) (Catimel et al. 1993; PMID： 8228269). This antibody was used for detection of Glycophorin A by flow cytometry (Lansdorp et al., 1992; PMID： 1375263).
Antibody First Published
Bigbee et al. Monoclonal antibodies specific for the M- and N-forms of human glycophorin A Mol Immunol. 1983 Dec;20(12)：1353-62. PMID：6197636
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
