Cat. No.
MABL-1711
Application
immunoblot, inhibition assay, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
M2A1
From
Recombinant Antibody
Specificity
M2A1 binds specifically to the M antigen on human erythrocytes, and shows no reactivity to N antigen. The epitope for this antibody includes the amino-terminal serine residue and sialic acid residues of glycophorin A. The reactivity of M2A1 for M antigen is pH- and salt-dependent (optimum ~ pH8-9). Blood group antigen M is located on the major sialoglycoprotein (glycophorin A) of red blood cells.
Alternative Names
CD235a; M sialoglycoprotein; PAS-2
UniProt
P02724
Immunogen
M2A1 was prepared by immunzing BALB/c mice with human red blood cells of the M phenotype, and was screened by testing for anti-M activity.
Application Notes
M2A1 can bind to M antigen of glycophorin A and can be used in ELISAs, immunoblots and modified M glycoprotein can be used for inhibition tests.
Antibody First Published
Jaskiewicz et al. Characterization of the epitope recognized by a monoclonal antibody highly specific for blood group M antigen. Transfusion. 1990 Mar-Apr;30(3)：230-5. PMID：1690467
Note on publication
Describes the generation and characterization of M2A1 antibody against M antigen.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
