Cat. No.
MABL-1712
Application
WB, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
JC159
From
Recombinant Antibody
Specificity
This antibody is specific to Glycophorin-A and does not recognise Glycophorin-B. Glycophorin-A is a sialoglycoprotein and a crucial element of the erythrocytes' membrane. Part of it is a receptor for certain viruses, such as influenza and hepatitis A.
Alternative Names
Glycophorin A; Glycophorin-A; MN sialoglycoprotein; PAS-2; Sialoglycoprotein alpha; CD235a; GPA; GYPA; glycophorin Erik; glycophorin MiI; glycophorin MiIII; glycophorin MiV; glycophorin MiX; glycophorin SAT; glycophorin Sta type C; GPErik; GpMiIII; GPSAT; HGpMiIII; HGpMiV; HGpMiX; HGpMiXI; HGpSta(C)
UniProt
P02724
Immunogen
This antibody was raised by immunising BALB/c mice against membrane preparation from splenic hairy cell leukaemia cells.
Application Notes
JC159 antibody may be used in experiments where detection of erythrocytes is needed. One group successfully used it to visualise haemorrhage in tissue samples when studying neuropathological features present in fatal malaria (Medana et al., 2002). Another group utilisied this antibody to identify redblood cell residues in human soft tissue lesions decomposed in air and water from formalin-fixed, paraffin-embedded skin samples in order to improve the uderstanding and assessment of cadaver-investigation in criminology (Taborelli et al., 2011). Finally, Pich et al. (2009) performed immunohistochemical analysis of bone marrow samples with this antibody when investigating the potential role of JAK2V617F mutation in myeloproliferative type of chronic myelomonocyticleukaemia.
Antibody First Published
Erber et al. A new monoclonal antibody (JC159) that detects glycophorin A for the diagnosis of erythroleukaemia Hematol Rev 5 (1991)： 113-20.
Note on publication
This article characterises JC159 antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
