Cat. No.
MABL-1715
Application
in vivo, neutralizing, SPR
Isotype
Engineer antibody
Species Reactivity
Bovine Schmallenberg virus
Clone No.
1C11
From
Recombinant Antibody
Specificity
The antibody is specific for the envelopment polyprotein of SBV. It binds to a conformational epitope on the apical end of the head domain near the N686 glycosylation site. Its epitope has an area of 907 A2 and is centered on the third disulfide bond, with contributions from the regions around helices h8–h10 and h12–h13.
Alternative Names
Gc; SBV; M polyprotein; Glycoprotein G1; Envelopment polyprotein
UniProt
H2AM12
Immunogen
The original antibody was generated by immunizing BALB/c mice with SBV strain BH80/11.
Application Notes
The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody reacted with SBV by indirect immunofluorescence (Wernike et al., 2015; PMID： 25889366). The binding affinity of the original format of the antibody to the recombinant SBV head–stalk construct was measured by surface plasmon resonances (KD = 5.4 ± 0.3 nM). The original format of the antibody neutralized SBV on Vero cells in a focus reduction neutralization (FRNT) assay with ND50 = 0.15 ± 0.01 µM. The scFv format of the antibody was co-crystallized in complex with the SBV Gc head domain. The original format of the antibody could protect IFNAR−/− mice from lethal SBV infection in vivo (Hellert et al., 2019; PMID： 30787296).
Antibody First Published
Wernike et al. A novel panel of monoclonal antibodies against Schmallenberg virus nucleoprotein and glycoprotein Gc allows specific orthobunyavirus detection and reveals antigenic differences Vet Res. 2015; 46: 27. Published online 2015 Mar 11. doi: 10.1186/s13567- 015-0165-4 PMID:25889366
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
