Anti-Glycoprotein D mAbs [HSV8](MABL-1716)

MABL-1716

in vivo, inhibition, IP, neutralizing, WB, ELISA, IF

Engineer antibody

Herpes simplex virus (HSV)

HSV8

Recombinant Antibody

The antibody binds to glycoprotein D (epitope located between residues 234 and 275).

gD; Envelope glycoprotein D

Q69091

The original antibody was derived from a human antibody library displayed on phage, and identified by panning with herpes simplex virus proteins.

Screening of the phage display for significant binding towards HSV-1 and HSV-2 was done using ELISA; The Fab supernates were tested in immunofluorescence studies with virus infected cells. The protein recognized by the Fab was identified via immunoprecipitation. Western blot confirmed specificity Fab for gD. Fab fragment neutralizes infectivity and prevents cell-to-cell transmission of herpes simplex viruses 1 and 2. The Fab fragment neutralized HSV-1 at 0.25 ug/ml (50% reduction) and HSV-2 at 0.05 ug/ml. This Fab also inhibited plaque formation when applied to virus-infected monolayers, completely abolishing HSV-2 plaque development at 25 ug/ml 72 hours postinfection, indicating the ability of the Fab to prevent cell-to-cell spread of virus (Burioni et al, 1994; PMID:8278393). In vivo experiments showed that the antibody (IgG1) reduced the mortality rate in mice when administrated before HSV type 1 infection and it prolonged survival times when administered up to 24 hours postinfection (Sanna et al, 1996; PMID:8553581). The epitope mapping of this antibody was done using competition ELISA. The antibody demonstrated potent activities against cell-associated virus in a syncytium inhibition assay (De Logu et al, 1998; PMID:9774565). IgG1, Fab and F(ab)2 fragments were tested against HSV-2 in mice in vivo and they were approximately equally protective (Zeitlin et al, 1996; PMID:8918548).

Burioni et al. Recombinant human Fab to glycoprotein D neutralizes infectivity and prevents cell-to-cell transmission of herpes simplex viruses 1 and 2 in vitro. Proc Natl Acad Sci U S A. 1994 Jan 4; 91(1): 355–359. PMID:8278393

The article describes the generation and characterization of the antibody.

100 μg Purified antibody.

1 mg/ml.

Protein A affinity purified

PBS with 0.02% Proclin 300.

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.