Cat. No.
MABL-1717
Application
Blocking, IP, ELISA
Isotype
Engineer antibody
Species Reactivity
Herpes Simplex Virus
Clone No.
E317
From
Recombinant Antibody
Specificity
This antibody binds specifically to the glycoprotein D of HSV. The epitope core structure maps to the external surface of gD, corresponding to the binding sites of two receptors, herpesvirus entry mediator (HVEM) and nectin-1, which mediate HSV infection. E317 directly recognizes the gD-nectin-1 interface and occludes the HVEM contact site of gD to block its binding to either receptor. The binding of E317 to gD also prohibits the formation of the N-terminal hairpin of gD for HVEM recognition. The major E317-binding site on gD overlaps with either the nectin-1-binding residues or the neutralizing antigenic sites identified thus far (Tyr38, Asp215, Arg222 and Phe223). The epitopes of gD for E317 binding are highly conserved between two types of human herpesvirus (HSV-1 and HSV-2).
Alternative Names
Envelope glycoprotein D; gD
UniProt
Q69091
Immunogen
Herpes simplex virus 1.
Application Notes
This antibody binds to glycoprotein D of HSV and blocks its intereaction with human cellular receptors. Glycoprotein D binds to cell entry receptors TNFRSF14/HVEM and others, triggering fusion with the host membrane.
Antibody First Published
Lee et al. Structural basis for the antibody neutralization of herpes simplex virus. Acta Cystallography Section D Biological Crystallography 2013; 69(Pt19):1935-1945 PMID:24100313
Note on publication
Describes the generation of a monoclonal antibody against HSV glycoprotein D and subsequent crystallisation as well as a discussion of its potential therapeutic use.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
