Cat. No.
MABL-1719
Application
crystallization, in vitro, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
314.3
From
Recombinant Antibody
Specificity
The antibody recognizes the N terminus of the GPIIb beta propeller domain only when soluble quinine is present.
Alternative Names
Integrin beta-3; Platelet membrane glycoprotein IIIa; GPIIIa; CD61; integrin αIIbβ3
UniProt
P05106
Immunogen
The original antibody was generated by immunizing BALB/c mice with a mixture of quinine- linked GPIIb/IIIa, unlinked GPIIb/IIIa, and soluble quinine.
Application Notes
The antibody reacted with normal human platelets in the presence of quinine, as shown by flow cytometry. The reactions of the antibody with CHO cells expressing all-human GPIIb/IIIa (H/H), all-rat GPIIb/IIIa (R/R), or human/rat mixed GPIIb/IIIa integrins (H/R, R/H) were studied by flow cytometry.Quinine dependent antibody binding occurred with constructs H/H and H/R, but not with R/R or R/H (Bougie et al., 2009; PMID: 18948570). The crystal structures of the Fab fragment and its complex with quinine were determined. Quinine induced structural modifications of the Fab fragment; the authors propose that quinine remodels the Fab paratope and enables binding to the platelet GPIIb/IIIa. The monovalent KD values for quinine binding to the IgG fragment measured was 24 nM (Zhu et al., 2015; PMID: 26282540).
Antibody First Published
Bougie et al. Quinine-dependent, platelet-reactive monoclonals mimic antibodies found in patients with quinine-induced immune thrombocytopenia. Blood. 2009 Jan 29; 113(5): 1105–1111. PMID:18948570
Note on publication
The paper describes the generation and characterization of the antibody. The antibody is employed in studies to define the molecular basis for drug-dependent antibody binding and platelet destruction in DITP.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
