Cat. No.
MABL-1723
Application
WB, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
4C11
From
Recombinant Antibody
Specificity
This antibody binds human glypican 3 (GPC3).Glypican-3 is an oncofetal antigen that belongs to the glypican family of glycosyl-phosphatidylinositol-anchored heparin sulfate proteoglycans. It negatively regulates the hedgehog signaling pathway and positively regulates the canonical Wnt signaling pathway. Multiple studies have revealed that GPC-3 is overexpressed in hepatocellular carcinoma (HCC).
Alternative Names
GPC3; Glypican-3; GPC-3; GTR2-2; MXR7; OCI5; DGSX; Intestinal protein OCI-5; CCTCC No: C2018156.
UniProt
P51654
Immunogen
The original antibody was generated by immunizing BLAB/c mice with recombinant N- terminal GPC3 antigen fragment emulsified in Freund's adjuvant.
Application Notes
The initial binding characterization of this antibody was done using ELISA. This antibody can identify human glypican 3 expressed on the Huh7, LM3, HepG2, PLC, PVTT and SMMC-7721 (7721) cell lysates in a western blot assay. This antibody was also used in the immunohistochemical staining of human liver cancer tissues (CN111040036).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
