Cat. No.
MABL-1722
Application
ELISA, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Rat, Human, Cynomolgus Monkey
Clone No.
15G1
From
Recombinant Antibody
Specificity
This antibody binds an epitope between amino acids 420 to 470 of the human glypican 3 (GPC3). This antibody cross reacts with cynomolgus monkey and rat GPC3. Glypican-3 is an oncofetal antigen that belongs to the glypican family of glycosyl-phosphatidylinositol-anchored heparin sulfate proteoglycans. It negatively regulates the hedgehog signaling pathway and positively regulates the canonical Wnt signaling pathway. Multiple studies have revealed that GPC-3 is overexpressed in hepatocellular carcinoma (HCC).
Alternative Names
GPC3; Glypican-3; GPC-3; GTR2-2; MXR7; OCI5; DGSX; Intestinal protein OCI-5
UniProt
P51654
Immunogen
The original antibody was generated by immunizing BALB/c mice with a recombinant human GPC3 extracellular domain (ECD, amino acids of 1-547) fused to a C-terminal Flag (RADYKDDDDK).
Application Notes
This antibody can bind human glypican 3 expressed on the surface of 293S cells, HepG2 X1 cells and JHH7 cells in a flow cytometric assay. This antibody reacts with human rat and cynomolgus monkey GPC3 in a flow cytometric assay. The binding characterization of this antibody to huGPC3, cynoGPC3, and HepG2 cells was done using ELISA and immunohistochemistry. (US9926377).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
