Cat. No.
MABL-1727
Application
IM, ThT fluorescence assay, WB, EM
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
4396
From
Recombinant Antibody
Specificity
This antibody is specific for human GM1-Aβ.
Alternative Names
Gaβ; GM1 ganglioside-bound amyloid β-protein; 4396C
UniProt
Immunogen
The original antibody was generated by immunizing mice with purified human brain-derived GM1-Aβ.
Application Notes
The original mouse IgM antibody was developed and tested to specifically recognize Aβ bound to GM1 (GM1-Aβ). A subsequent version, 4396C, an IgG2a subtype chimeric antibody, was generated and evaluated. The chimeric antibody retained its specificity for GM1-Aβ, as demonstrated by electron microscopy (EM), where it specifically labeled GM1-containing liposomes incubated with Aβ; Western blotting (WB), which confirmed the antibody’s specificity for GM1-Aβ; a Thioflavin T (ThT) assay, which showed that the antibody inhibited amyloid fibril formation in a dose-dependent manner when incubated with GM1-containing liposomes and Aβ; and immunoelectron microscopy (IM), where it effectively bound to newly formed amyloid fibrils in the presence of synthetic Aβ and GM1-containing liposomes (US7339035B2). The chimeric antibody was assessed in three polymerization systems: (1) polymerization initiated by adding Aβ fibril fragment, (2) Aβ40 polymerization initiated from GAβ40, and (3) Aβ42 polymerization initiated from GAβ42. It exhibited suppression of the Aβ extension (US7741448B2).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
