Cat. No.
MABL-1749
Application
ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Cryptococcus neoformans
Clone No.
2E 9
From
Recombinant Antibody
Specificity
This antibody is specific for GXM or glucuronoxylomannan from Cryptococcus neoformans. GXM is a polysaccharide expressed on the surface of C. neoformans. This antibody binds an epitope of C. neoformans that is different from the epitope of another anti-GXM antibody 2H1.
Alternative Names
Glucuronoxylomannan; Cryptococcus neoformans capsular polysaccharide; CNPS
UniProt
Immunogen
The original antibody was generated from the peripheral blood lymphocytes of glucuronoxylomannan-tetanus toxoid conjugate (GXM-TT)-immunized individual.
Application Notes
This antibody was used for the immunofluorescent labelling of C. neoformans serotype D and A. This antibody can be used in combination with another anti-GXM antibody 2H1 for detection of all four serotypes of C. neoformans GXM using sandwich ELISA. The antibody 2H1 is used as capture antibody and 2E9 is used as a detection antibody (PMID: 7622223).
Antibody First Published
Pirofski et al. Analysis of human monoclonal antibodies elicited by vaccination with a Cryptococcus neoformans glucuronoxylomannan capsular polysaccharide vaccine. Infect Immun. 1995 Aug;63(8):3005-14. PMID:7622223
Note on publication
Describes the generation of two human antibodies from the peripheral blood lymphocytes (PBLs) of a GXMTT-immunized individual.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
