Cat. No.
MABL-1750
Application
therapeutic, ELISA
Isotype
Engineer antibody
Species Reactivity
Cryptococcus neoformans
Clone No.
2H1
From
Recombinant Antibody
Specificity
This antibody is specific for GXM from C.neoformans. GXM is a polysacharide experssed on the surface of c.neoformans.
Alternative Names
Glucuronoxylomannan; Cryptococcus neoformans capsular polysaccharide; CNPS
UniProt
Immunogen
The original mouse version of this antibody was raised by infecting balb/c mice with C. neoformans and then immunizing them with glucuronoxylomannan-tetanus toxoid conjugate (GXM-TT).
Application Notes
To characterize the mouse version of this antibody an ELISA was performed on native and de-O-acetylated serotype A GMX using the mouse version of this antibody (Casadevall et al, 1992; pmid:1583327). In research on the efficacy of the mouse version of this antibody against diverse strains of Cryptococcus neoformans. This antibody showed the ability to reduce the amount of yeast cells present in the organs of mice (Mukherjee et al, 1995; pmid:7642262).
Antibody First Published
Casadevall et al. Antibodies elicited by a Cryptococcus neoformans-tetanus toxoid conjugate vaccine have the same specificity as those elicited in infection J Infect Dis. 1992 Jun;165(6):1086-93. PMID:1583327
Note on publication
The antibody responses of BALB/c mice to serotype A Cryptococcus neoformans capsular polysaccharide (CNPS) were compared after cryptococcal infection and immunization with a serotype A glucuronoxylomannan-tetanus toxoid conjugate.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
