Cat. No.
MABL-1754
Application
inhibition, IP, FC, IF
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
AF6-88.5.5.3
From
Recombinant Antibody
Specificity
This antibody is specific for mouse H-2 class I histocompatibility antigen. H-2 class I histocompatibility antigen is involved in the presentation of foreign antigens to the immune system.
Alternative Names
H-2 class I histocompatibility antigen, K-B alpha chain; H-2K(B); H2-K1; H2-K
UniProt
P01901
Immunogen
This antibody was produced by the fusion of mouse myeloma Sp2/0 cells with spleen cells from a rat, that was immunized with the H-2 class I histocompatibility antigen.
Application Notes
Cloned mouse Th cells L2, grown on CBA/J stimulating spleen cells were used for flow cytometry using this antibody (Wall et al, 1983; pmid:6193171). The inhibition of 125I-7 1-54.3 binding to mouse spleen cel was tested using this antibody (Wall et al, 1983; pmid:6193171). Cells isolated from the spleen, BM, and peripheral blood of transplanted and control animals were assayed by flow cytometry using this antibody (Bjornson et al, 1999; pmid:9915700). Mouse spleen leukocytes were analyzed in flow cytometry using this antibody (Xie et al, 2006; pmid:17050600). Plenic lymphocytes derived from transgenic mice expressing wild-type (WT) H-2Kb, ΔY or Δ7. H-2Kb were used for immunoprecipitation. In this procedure the proteins were immunoprecipitated using this antibody (Lizée et al, 2003; pmid:14566337). Peripheral blood leukocytes from mice were used in a flow cytometry assay using this antibody (Lizée et al, 2003; pmid:14566337). Immunofluorescence was preformed on mouse mature spleen-derived DCs using this antibody (Lizée et al, 2003; pmid:14566337). M1 cells, a clonal isolate derived from B16-F10 murine melanoma cells, were immunostained using this antibody (Hauser et al, 1993; pmid:8467514).
Antibody First Published
Wall et al. INHIBITION OF PROLIFERATION OF MIS- AND la-REACTIVE CLONED T CELLS BY A MONOCLONAL ANTIBODY AGAINST A DETERMINANT SHARED BY I-A AND LE1 J Immunol. 1983 Sep;131(3):1056-64. PMID:6193171
Note on publication
The interaction between cloned amplifier T cells and stimulating cells was probed with the use of a monoclonal antibody selected for its ability to inhibit stimulation of MIS- and la-reactive T cells.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
