Cat. No.
MABL-1755
Application
functional assay, FC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
AF3-12.1.3
From
Recombinant Antibody
Specificity
This antibody is specific for the mouse H-2Kk MHC antigen. H-2Kk MHC is involved in the presentation of foreign antigens to the immune system.
Alternative Names
H-2 class I histocompatibility antigen, K-K alpha chain; H2-K1; H-2K(K); H2-K
UniProt
P04223
Immunogen
The antibody was raised against the mouse H-2Kk MHC antigen.
Application Notes
The passive transfer of monoclonal antibodies was tested using this antibody. This experiment was preformed on cardiac allograft recipients (Rahimi et al, 2004; pmid:14961984). It was tested whether this antibody could activate the complement. It could not (Takenaka et al, 2012; pmid:22980951). Bone marrow plug grafts were removed from the kidney capsule and used for flow cytometry using this antibody. The test animal used was a mouse (Dai et al, 2017; pmid:28783664). Mouse single-cell suspensions from the thymus, lymph nodes, and spleen were used for flow cytometry with this antibody (Martínez-Riaño et al, 2019; pmid:31324740).
Antibody First Published
Rahimi et al. Non-complement- and complement-activating antibodies synergize to cause rejection of cardiac allografts Am J Transplant. 2004 Mar;4(3):326-34. PMID:14961984
Note on publication
Alloantibodies (AlloAbs) are a clinically significant component of the immune response to organ transplants. The effect of these antibodies was tested on mice.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
