Cat. No.
MABL-1761
Application
CHIP, ChIP-seq, IP, IP-MS, WB, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
304M3-B
From
Recombinant Antibody
Specificity
This antibody is highly specific for Histone H3 trimethylated at lysine 4. It has been tested on human histone H3 (K562 cells), but owing to the high conservation of histones, is expected to cross-react with many other species.
Alternative Names
trimethyl-Histone H3 (Lys4); histone H3; H3K4; Trimethylated Histone H3; Trimethylated Lys; Trimethylated Lys4; Histone H3 trimethylated at Lysine 4; post translational modification; histone post-translational modification
UniProt
B2R4P9
Immunogen
The biotinylated peptide from histone H3 ARTKQTARKSTG-GYCD. The last four amino acids GYCD were added for biotinylation purposes.
Application Notes
This antibody has been used in IP-studies and works in immunofluorescence and Western Blot. In an long-neck scFv-Fc-fusion format been shown to exhibit a high degree of specificity owing to an antigen-clasping binding mechanism (see Hattori et al. 2016).
Antibody First Published
Hattori et al. Antigen clasping by two antigen-binding sites of an exceptionally specific antibody for histone methylation. Proc Natl Acad Sci U S A. 2016 Feb 23;113(8):2092- 7 PMID:26862167
Note on publication
Describes the characterization of the specificty of this antibody through antigen- clasping.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
