Cat. No.
MABL-1762
Application
IP, WB, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
scFv-58F
From
Recombinant Antibody
Specificity
The original antibody is strongly specificity for the acetylated lysine 9 of Histone H3. It does not cross-react with any other acetylated, phosphorylated, or methylated residue of histone H3, nor with any other histone protein.
Alternative Names
acetylated lysine 9 of histone H3
UniProt
Immunogen
The original antibody was isolated from a mouse naïve ScFv library by panning against the acetylated lysine 9 of Histone H3.
Application Notes
The specificity of the original format of the antibody for acetylated lysine 9 of histone H3 was confirmed by ELISA analysis. The original format of the antibody detected acetylated lysine 9 of histone H3 by western blot analysis. The original format of the antibody could immunoprecipitate recombinant native acetylated histone H3 in vitro. The original format of the antibody-bound yeast acetylated H3 in vivo, as it was able to immunoprecipitate it after cell lysis and chromatin sonication. Moreover, the intracellular expressing antibody was able to reduce acetylated histone availability in chromatin. Immunofluorescence was performed on HeLa cells using this antibody. The antibody strongly decorates nuclei of HeLa cells, with the exclusion of perinuclear (lamin positive) regions, indicative of euchromatic staining (US11137393B2).
Antibody First Published
Chirichella et al. Post-translational selective intracellular silencing of acetylated proteins with de novo selected intrabodies. Nat Methods. 2017 Mar;14(3):279-282. doi: 10.1038/nmeth.4144. Epub 2017 Jan 16. PMID:28092690
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
