Anti-HA tag mAbs [12CA5](MABL-1771)

MABL-1771

IP, WB, IF

Engineer antibody

Species independent

12CA5

Recombinant Antibody

This antibody is specific for the HA-tag. HA contains a GTPase activator for the Rho-type GTPases (RhoGAP) domain that would be able to negatively regulate the actin cytoskeleton as well as cell spreading. However, also contains N-terminally a BAR-domin which is able to play an autoinhibitory effect on this RhoGAP activity.

Rho GTPase-activating protein 45; Arhgap45; Minor histocompatibility protein HA-1

Q3TBD2

This antibody was raised by immunizing balb/c mice with a 9 amino acid petide to influenza virus hemagglutinin.

The mouse version of this antibody has shown the ability to immunoprecipitate HA bound to BAX from cell lysates (Cheng et al, 1996; pmid:8596636). HA tagged SIM1 was immoprecipitated using the mouse version of this antibody. Other proteins present that lacked the HA tag were not immunoprecipitated (Michaud et al, 2000; pmid:10640708). A western blot has detected HA bound to PI4P5K using the mouse version of this antibody (Shibasaki et al, 1997; pmid:9065410). The HOXC6 protein bound to a HA-tag was immunoprecipitated using the mouse version of this antibody (McCabe et al, 2008; pmid:18339881). Cells expressing MLS2 fused to the HA epitope were stained using the mouse version of this antibody. Further the MSL2-HA protein was immuoprecipitated using this antibody (Smith et al, 2000; pmid:10594033). The Pat1 gene tagged with HA can be immunoprecipitated from lysed yeast cells using the mouse version of this antibody. Furthermore, the mouse version of this antibody can detect pat1-HA in a western blot. The western blot was preformed to determine the effect of topoisomerase II activity on chromosome segregation (Wang et al, 1996; pmid:8972867). HA tagged proteins can be stained using the mouse version of this antibody. Furthermore, the SPOP protein bound to a HA tag is able to be detected by the mouse version of this antibody on a western blot. The western blot was preformed to identify a novel antigen found in serum from a sclerodoma patient (Nagai et al, 1997; pmid:9414087).

Cheng et al. Bax-independent inhibition of apoptosis by Bcl-XL Nature. 1996 Feb 8;379(6565):554-6. PMID:8596636

Using site-specific mutagenesis,it is shown here that the amino-acid residues critical for protection of cells by Bcl-XL against Sindbis virus-induced apoptosis are clustered within the Bcl-2- homology regions 1 and 2 (BH1 and BH2 regions).

100 μg Purified antibody.

1 mg/ml.

Protein A affinity purified

PBS with 0.02% Proclin 300.

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.