Cat. No.
MABL-1770
Application
SPR, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Influenza A (H5N1)
Clone No.
A3
From
Recombinant Antibody
Specificity
The antibody specifically recognizes the sequence (NS)PQRER observed at the cleavage site of the hemagglutinin protein of the H5N1 avian influenza virus. The antibody does not cross-react with the HA protein of H1N1 or H7N7 strain.
Alternative Names
Hemagglutinin Cleavage Site; CS HA; Avian Influenza Virus; H5N1
UniProt
A5A5M5
Immunogen
The original antibody was generated by immunizing mice with the cleavage site (CS) peptide of hemagglutinin protein, followed by antibody library construction and phage display-based selection.
Application Notes
The specificity of this antibody (Fab) was confirmed by ELISA. The epitope mapping of this antibody was done using ELISA. The binding affinity of the Fab fragment towards the cleavage site peptide of HA derived from the HPAI H5N1 virus was found to be Kd= 0,80 nM using surface plasmon resonance. The clone was used for detection of the cleavage site peptide on an open sandwich ELISA, reaching the lower limit of detection (LOD) in the range of 1 to 10 ng/ml. This antibody was also used in the immunofluorescent staining of Madin-Darby canine kidney cells infected with HPAI H5N1 (Dong et al, 2013; pmid:23577205).
Antibody First Published
Dong et al. Isolation of recombinant phage antibodies targeting the hemagglutinin cleavage site of highly pathogenic avian influenza virus PLoS One. 2013;8(4):e61158. PMID:23577205
Note on publication
The paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
