Cat. No.
MABL-1776
Application
inhibition, neutralization, WB, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
KM3566
From
Recombinant Antibody
Specificity
The antibody is specific for HB-EGF.
Alternative Names
HBEGF; Proheparin-binding EGF-like growth factor; Heparin-binding EGF- like growth factor; Diphtheria toxin receptor; DT-R
UniProt
Q99075
Immunogen
The original antibody was generated by immunizing mice with human HB-EGF.
Application Notes
The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody detected the recombinant secretory human HB-EGF by western blot analysis. The HB-EGF- EGFR binding inhibitory activity of the original antibody was examined using 32D/EGFR cell and biotin- labeled HB-EGF. The original antibody concentration-dependently inhibited binding of biotinylated HB-EGF to EGFR. Further, it could also inhibit binding of HB-EGF with EGFR. The neutralizing activity of the original antibody was examined by a cell growth inhibition assay which used an HB-EGF-dependent cell. The original antibody showed HB-EGF-dependent cell growth inhibition activity for the cell line 32D/EGFR. The original antibody bound to the membrane type and cell membrane-bound HB-EGF of cancer cell lines (human gastric cancer cell line MKN-28, human ovarian cancer cell line ES-2, human breast cancer cell line MDA-MB-231).The binding affinity of the original format of the antibody to human HB-EGF was measured by surface plasmon resonance (Ka= 2.7 1/Ms). The original antibody specifically bound to the T cell leukemia and acute myelogenous leukemia cell lines expressing HB-EGF as shown by FC (US7851601B2).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
