Cat. No.
MABL-1777
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
1G8
From
Recombinant Antibody
Specificity
This antibody binds human hemoglobin glycated at the N-terminal valine of the hemoglobin β chain and does not cross react with non-glycated hemoglobin. Glycated hemoglobin is formed by the conjugation of a hexose sugar to the beta-chain of hemoglobin. Glycosylated hemoglobin A1c (HbA1c) levels are used as an indicator of average plasma glucose concentration in diabetic patients. Levels of more than 48 mmol/mol (6.5%) are used as a criterion for the diagnosis of diabetes. Higher levels of HbA1c are also associated with cardiovascular disease.
Alternative Names
GHb; A1C; HBB; hemoglobin A1c; glycosylated hemoglobin HbA1c; glycosylated hemoglobin; glycohemoglobin; glycated hemoglobin; haemoglobin; haemoglobin A1c; Hemoglobin subunit beta; Beta-globin; Hemoglobin beta chain
UniProt
P68871
Immunogen
The original antibody was generated by immunizing BALB/c mice with synthetic N-terminal glycopeptide (VHLTPEC) and KLH conjugate.
Application Notes
This antibody is recommended for detection of HbA1c levels in plasma and blood samples. The binding reactivity of this antibody towards HbA1c and non-glycated hemoglobin was done using indirect ELISA. The binding reactivity of this antibody to HbA1c was compared to commercially available anti-HbA1c antibody BM300-F7C2 and it was reported that this antibody showed better specificity for HbA1c (CN110724671).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
