Cat. No.
MABL-1784
Application
immunotherapy, IP, neutralize, ELISA
Isotype
Engineer antibody
Species Reactivity
Hepatitis C virus
Clone No.
AR3A
From
Recombinant Antibody
Specificity
This antibody is specific for the HCV E2 gylcoprotein. This antibody can recognize soluble E2 and native folded E2 glycoprotein but not reduced or denatured protein.
Alternative Names
E2; Genome polyprotein; Envelope glycoprotein E2; NS1; gp68; gp70
UniProt
P27958
Immunogen
The originala antibody was isolated from a phage-display library generated from a donor chronically infected with HCV.
Application Notes
This broadly neutralizing anitbody binds the AR3 antigenic regions of the HCV envelope glycoprotein and is capable of neutralizing JFH-1, H77, H, OH8, UKN1 B12.16, J6, UKN2 A1.2, UKN2 B1.1, UKN4. 21.16, UKN5. 15.7 strains of HCV (PMID: 18064037, 22492964). The IgG1 version of AR3A inhibited the binding of E1-E2 complex to the virus co-receptor CD81 at nanomolar concentration suggesting that this antibody can block HCV interaction with CD81 and thereby inhibit infection. The binding specificity of this antibody for E2 glycoprotein was determined using ELISA. AR3A provided protective immunity in two out of five mice receiving the antibody. This (PMID:18064037). This antibody was used for the immunoprecipitation of E2 glycoprotien cell lysates (PMID: 18355410).
Antibody First Published
Law et al. Broadly neutralizing antibodies protect against hepatitis C virus quasispecies challenge. Nat Med. 2008 Jan;14(1):25-7. doi: 10.1038/nm1698. PMID:18064037
Note on publication
Describes the generation of antibodies against three different antigenic regions of the Hepatitis C virus.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
