Cat. No.
MABL-1783
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
HCV, Hepatitis C virus
Clone No.
19D9D6
From
Recombinant Antibody
Specificity
This antibody is specific for the protein sequence QIVGGVYLL located at residues 29-37 of the HCV core protein. Hepatitis C virus (HCV) is the primary cause of hepatitis transmitted through blood transfusion. It is an enveloped virus that contains a single-stranded, positive-sense RNA genome of approximately 9600 nucleosides. This genome encodes a polyprotein of 3011 amino acids, which undergoes post-translational cleavage to form both structural and nonstructural proteins. Among the structural proteins, the core protein is derived from the amino terminus of the viral polypeptide (amino acids 1-191).
Alternative Names
Hepatitis C virus core protein
UniProt
Immunogen
The original antibody was generated by immunizing BALB/c JYco mice with a truncated recombinant protein corresponding to the immunodominant region (residues 1–120) of the Hepatitis C Virus (HCV) purified nucleocapsid protein (HCV C120-GST fusion protein).
Application Notes
This antibody was used for an indirect ELISA to detect anti-HCV core response in human sera, an ELISA competition assays to assess the interaction between monoclonal antibodies and anticore human sera, a sandwich ELISA for the capture and detection of viral core antigen in chronic HCV patient sera, and a sandwich ELISA for the detection of recombinant core protein (Jolivet-Reynaud et al., 1998; PMID: 9829633). The crystal structure of the Fab version of this antibody was solved in complex with an HCV core protein-derived peptide (residues 13-40), revealing the hydrophobic nature of the recognized epitope (Ménez et al., 2003; PMID: 12574359).
Antibody First Published
Jolivet-Reynaud et al. HCV core immunodominant region analysis using mouse monoclonal antibodies and human sera: characterization of major epitopes useful for antigen detection J Med Virol. 1998 Dec;56(4):300-9. doi: 10.1002/(sici)1096-9071(199812)56:4300:aid- jmv3>3.0.co;2-8 PMID:9829633
Note on publication
The original publication focuses on the generation of monoclonal antibodies (MAbs) against the immunodominant region of the hepatitis C virus (HCV) core protein and the characterization of major epitopes within this region that are useful for antigen detection.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
