Cat. No.
MABL-1792
Application
crystallography, in vitro, SPR, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Vibrio cholerae, Escherichia coli
Clone No.
anti-CTP3-TE33
From
Recombinant Antibody
Specificity
The epitope of this antibody is the sequence: VEVPGSQHIDSQKKA from Cholera toxin peptide 3 (CTP3)
Alternative Names
CTB; eltB; ltpB; CTP3; LT-B; LTP-B; ctxB; porcine; Cholera enterotoxin B- subunit; Cholera enterotoxin subunit B; Cholera toxin B protein
UniProt
P32890; Q57193
Immunogen
The original antibody was raised by immunizing a Balb/c mouse with amino acid residues 50- 64 of the B subunit of cholera toxin coupled to tetanus toxoid
Application Notes
The original antibody was used for fluorescence quenching on DNP-CTP3. This experiment was performed to determine the binding affinity of this antibody, which is 1.2 x 10^6 M^-1. The antibody was used for a radioimmunoassay on cholera toxin and H-LT, a heat-labile toxin produced by enterotoxigenic strains of E. coli isolated from humans. The antibody bound to both antigens. The antibody was used for a western blot on cholera toxin and H-LT, a heat-labile toxin produced by enterotoxigenic strains of E. coli isolated from humans. Which showed the same results as the radioimmunoassay (Anglister et al., 1988; PMID:2450576). The structure of this antibody was determined using NMR measurements (Zilber et al., 1990; PMID:2271636). The structure of this antibody was determined using X-ray crystallography (Shoham, 1993; PMID:7690406). This antibody was used for Phage-colony dot immunoblotting. This antibody was used for an ELISA on synthetic peptide VEVPGSQHIDSQKKA. Further the antibody was used for surface plasmon resonance on the same peptide (Otte et al., 2006; PMID:16273596).
Antibody First Published
Anglister et al. NMR study of the complexes between a synthetic peptide derived from the B subunit of cholera toxin and three monoclonal antibodies against it Biochemistry. 1988 Jan 26;27(2):717-24. PMID:2450576
Note on publication
In the original paper the contact interactions between a synthetic peptide and three different anti-peptide monoclonal antibodies have been studied by nuclear magnetic resonance (NMR).
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
